West Nile Virus (WNV) is responsible for West Nile Fever, a viral infection typically spread by mosquito bites. The infection is generally asymptomatic but can cause meningitis and encephalitis in immunocompromised patients and older people. WNV detection by using a molecular method is crucial to quickly establish an early diagnosis of a West Nile Virus infection.
WNV ELITe MGB® Kit is a one step, reverse-transcription and real-time PCR assay for the detection and quantification of WNV genomic RNA. The assay is CE-IVD validated on a large range of sample types with diverse conventional extraction and PCR systems.
|Conserved targets||· NS5 region of WNV gene and MS2 phage genomic RNA as an internal control|
|One-step format||· Reverse transcription and PCR amplification within the same run|
|Best-in-class PCR technology||· ELITe MGB probes designed with Minor Groove Binder, Superbases® and Eclipse® Dark Quencher|
|Samples||· Whole blood, cerebrospinal fluid and urine|
|Instruments||· Extraction: ELITe Star
· Thermal cyclers: ABI 7300 Real Time PCR system and ABI 7500 series
|High performance||· 96.4% clinical sensitivity and 100% clinical specificity|
|Complete transplant menu||· Complete transplant pathogen monitoring range|
|WNV ELITe MGB Kit||WNV PreMix: 2 tubes, 50 reactions each|
PCR MasterMix: 2 tubes, 50 reactions each
RT EnzymeMix: 2 tubes, 50 reactions each
|WNV ELITe Standard||2 sets of 4 levels, 8 reactions each||STD100PLD|
|WNV ELITe Positive Control||1 tube, 12 reactions||CTR100PLD|
|CTRCPE – Internal Control||4 tubes, 25 reactions each||CTRCPE|