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Unique Real-Time PCR technologies
Over the years, we have developed a comprehensive suite of PCR technologies, including the revolutionary Minor Groove Binder (MGB), to design superior sensitivity and specificity real-time PCR assays beneficial for laboratories, and clinicians .
|Pleiades probes are proprietary probes concentrating all ELITechGroup PCR technologies, designed with a Minor Groove Binder (MGB), Eclipse® Dark Quencher, Aquaphluor® dyes, and Superbases® • Very low background and high hybridization-triggered fluorescence • Unique quenching effects with the combined action of MGB and Eclipse® Dark quencher • Exceptional specificity due to a shorter length • Improve sensitivity by combination high hybridization signal with low and stable background • Melt-curve analysis capability
|MGB is a synthetic molecule attached to the 5’-end of MGB DNA probes, that binds in the minor groove of hybrid DNA molecules • Increase stability of hybrid DNA enabling the design of shorter probes for excellent specificity and sensitivity polymorphisms discrimination • Identify emerging mutations and reliably discriminate clinically important variants • Increase reproducibly in A-T and G-C rich regions • Enable reliable, clear and unambiguous melt-curve analysis
|Eclipse® DarkQuencher absorbs the fluorophore energy and dissipates it as heat; while a typical quencher re-emits part of the energy as light • Improve absorbance of fluorescence emitted from dye reagents • Decrease background level of fluorescence • Improve PCR assay sensitivity • Cover 4 spectra wavelengths
|Aquaphluor® dyes are fluorescent compounds that can re-emit light upon light excitation • Comprehensive range of 25 different colours from UV to far-red developed • Perfectly adapted to multiplexed assays
Superbases: Super A, Super T, Super G, and Super I
|Superbases are engineered nucleotides selectively used to optimize primers and probes design • Enhance A-T stability (e.g. rich A/T domains) • Reduces secondary structure (e.g. GG self-association) and fluorescence quenching • Confer close Tm value when paired with any bases
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