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Culture medium for the detection and isolation of Polymyxin-resistant bacteria strains

The SuperPolymyxin™ is a new selective culture medium for the early detection and isolation of Gram-negative bacteria with a reduced susceptibility to polymyxins. It was specially formulated to avoid swarming of Proteus spp. and to prevent contamination by Gram-positive bacteria and Fungi. The SuperPolymyxin will open new perspectives compared to the existing culture media containing high concentrations of colistin and which are known for screening intrinsic PR bacterial species (such as Serratia marcescens and Burkholderia cepacia) but are not adapted for screening isolates with acquired PR that may be of a low level.

  • Fast results in 3 hours Screening medium aimed to detect any Polymyxin-Resistant bacteria regardless of its resistance mechanism and of its level
  • Specially formulated to avoid swarming of Proteus spp. (naturally resistant) and to prevent contamination by Gram-positive bacteria and fungi
  • Critical in human medicine for detecting carriers and containing outbreaks due to resistant isolates
  • Useful in veterinary medicine for surveillance surveys
  • Easy to perform and highly sensitive

Catalogue Number(s)

Ref. 23005
Product Description: SUPERPOLYMYXIN
Number of Tests: TBD


This optimal screening medium is based on the eosin methylene blue (EMB) medium, which is selective for Gramnegative
bacteria. It contributes to a presumption of species identification by differentiating lactose fermenters (dark
blue-brown colonies) from non fermenters (colorless or light lavender). Moreover, differentiation of lactose fermenters
is possible with Escherichia coli colonies displaying a characteristic metallic green sheen and Enterobacter spp. and
Klebsiella spp. with brown, dark-centered, and mucoid colonies.
The optimal colistin concentration detects all polymyxin-resistant strains.
Daptomycin is used as an inhibitor of Enterococcus and Staphylococcus strains. Amphotericin B is used as an antifungal



Samples: Bacterial colonies, rectal swabs
Procedure: Direct streaking from rectal swab transport medium, preparation of a 0.5 McFarland suspension broth needed for colonies / Incubation 24-48h at 37°C / Aerobic conditions



  • 2.5 months shelf life in ready-to-use format
  • No need for a selective pre-enrichment medium
  • Incubation 24-48h
  • No equipment required