Hepatitis E (HEV) is an increasing cause of liver infection worldwide, and is generally asymptomatic or sometimes associated with severe symptoms, similar to those observed with hepatitis A. After solid organ transplantation, patients treated with immunosuppressive drugs are especially at high risk of developing HEV infection. Hepatitis E detection by molecular methods is important to diagnose and identify acute and chronic infections.
Hepatitis E ELITe MGB® Kit is a real-time PCR assay designed for the detection of Hepatitis E virus belonging to 1, 2, 3, 3 and 4 genotypes. The assay is intended to be used for research-use-only applications (RUO) and has been tested in combination with ELITe InGenius®, a fully automated sample-to-result solution.
|Conserved targets||· ORF-2 region of Hepatitis E gene and genomic RNA of MS2 phage gene as an internal control|
|One-step format||· Reverse transcription and PCR amplification within the same run|
|Best-in class PCR technology||· ELITe MGB probes designed with Minor Groove Binder, Superbases® and Eclipse® Dark Quencher|
|Integrated workflow||· Fully automated process for extraction, amplification and result analysis with ELITe InGenius|
|Eluate storage||· Additional testing including multiple PCR from the same eluate, retesting or archiving|
|Minimal hands-on time||· Less than 2 minutes per sample from extraction to result analysis|
|Complete transplant menu||· Complete transplant pathogen monitoring range|
|HEV ELITe MGB Kit||HEV PCR MasterMix: 2 tubes, 48 reactions each|
HEV PreMix: 2 tubes, 48 reactions each
RT EnzymeMix: 2 tubes, 48 reactions each
|HEV-ELITe Positive Control||2 tubes, 5 reactions each||CTR130ING|
|CTRCPE – Internal Control||4 tubes, 12 reactions each||CTRCPE|