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PRODUCT
ELI.H.A Candida
Indirect hemagglutination test for serodiagnosis of candidiasis.
ELI.H.A Candida enables the quantitative determination, by indirect hemagglutination, of serum antibodies present in patients suffering from candidiasis (Candida albicans).
- Cost-effective test
- Easy and ready-to-use
- High sensitivity and specificity
- Fast results for clinicians (2 hours)
- Simple visual reading, without expert interpretation
| Reference | Name | Quantity |
| 44600 | ELI.H.A Candida | 120 tests |
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Benefits
Intended use
Candidiasis are opportunistic infections caused by yeast-like fungi of the Candida genus. Candida albicans is the most frequently isolated fungus and is also the most pathogenic. Immunological diagnosis, via the detection of antibodies, is highly important in suspected invasive, visceral or septicemic Candida infections because the fungus cannot be directly isolated from a clinical sample. ELITech developed the ELI.H.A Candida, an indirect hemagglutination assay, for the quantitative determination of anti-Candida albicans antibodies in the serum. It is rapid, easy-to-use and easy-to-read with results available within 2 hours.
Principle
Sheep red blood cells are sensitized with Candida albicans antigen. Diluted serum is mixed with sensitized sheep red blood cells. If anti-Candida albicans antibodies are present in the serum, sensitized red blood cells will agglutinate, resulting in a cloudy red/brown deposit coating the well. In the absence of specific antibodies, sensitized red blood cells will not agglutinate, resulting in a ring-like deposit at the bottom of the well. Non-sensitized red blood cells are also available in the kit. They ensure the specificity of the reaction by allowing identifying any interference from the natural anti-sheep agglutinins (Forssman heteroantibodies, infectious mononucleosis antibodies…). The reaction is carried out in a U-microplate. Each kit allows 120 tests to be carried out or 20 reactions of 6 dilutions. Results are obtained within 2 hours and are reliable overnight.
Simple protocol
A single protocol for all our ELI.H.A kits.
- First serum dilution
- Microplate preparation
- Serial dilution of serum and preparation of the ‘serum control’ well
- Addition of sensitized RBC and non sensitized RBC
- Gently mix the plate and let stand 2 hours before reading
![eliha_methodology[2]](http://www.elitechgroup.com/wp-content/uploads/2015/01/eliha_methodology2.jpg)
The following procedures are therefore valid for all our ELI.H.A range:
Easy-to-read and easy-to-interpret results
![eliha_results[1]](http://www.elitechgroup.com/wp-content/uploads/2015/01/eliha_results13.jpg)
| Titer | Interpretation |
| <1:160 | Non significant reaction of a positive infection Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test |
| =1:160 | Doubtful reaction Possibility of a progressive candidiasis. The results must be interpreted by taking into account the full clinical picture. Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test. |
| ≥1:320 | Significant reaction in favor of progressive candidiasis |
Reagents and material
| Quantity | Description |
| 1 | R1: Vial of 2.4 mL of sensitized red blood cells |
| 1 | R2: Vial of 1 mL of non-sensitized red blood cells |
| 1 | BUF: Vial of 55 mL of phosphate buffer pH 7.2 |
| 1 | R3: Vial of 2 mL of adsorbent |
| 1 | CONTROL +: Vial of 0.2 mL of titrated positive control |
| 2 | CONTROL -: Vial of 0.2 mL of negative control |
| 2 | MICROPLATE: Microplate with a U-bottom |
Stability and storage
- The reagents are ready-to-use.
- All the reagents are stored at 2-8°C. Do not freeze.
- The results of the stability study indicate that the product has a stability of 24 months from its manufacture.
Material required but not provided
- Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured
- Contaminated waste containers
- Centrifuge
- Hemolysis tubes
Performance
| Sensitivity | Specificity |
| 85.4% | 97.9% |
Manufacturer: ELITech Microbio.
Product(s) intended for healthcare professionals.
Read the instructions on the label and/or instructions for use of the product(s).
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