PRODUCT

ELI.H.A Aspergillus

Indirect hemagglutination test for serodiagnosis of Aspergillosis.

ELI.H.A Aspergillus enables the quantitative determination, by indirect hemagglutination, of serum antibodies present in patients suffering from aspergillosis (Aspergillus fumigatus).

  • Liquid ready-to-use reagents
  • Cost-effective test
  • High sensitivity and specificity
  • Fast results for clinicians (2 hours)
  • Simple visual reading, without expert interpretation

 

Reference Name Quantity
44602 ELI.H.A Aspergillus 102 tests

 

ELI.H.A Aspergillus quantitative detection

Benefits

Intended use

  • Local conditions (preformed cavities, debrided pulmonary abscesses, impaired mucous membranes…);
  • General conditions (immunosuppression following major surgery or medical procedures: organ transplantation, immunosuppressive therapy, steroids, antibiotics…).

ELITechGroup developed the ELI.H.A Aspergillus, an indirect hemagglutination assay, for the quantitative determination of anti-Aspergillus fumigatus antibodies in the serum. It is rapid, easy-to-use and easy-to-read with results available within 2 hours.


Principle

Sheep red blood cells are sensitized with Aspergillus fumigatus antigen. Diluted serum is mixed with sensitized sheep red blood cells. If anti-Aspergillus fumigatus antibodies are present in the serum, sensitized red blood cells will agglutinate, resulting in a cloudy red/brown deposit coating the well. In the absence of specific antibodies, sensitized red blood cells will not agglutinate, resulting in a ring-like deposit at the bottom of the well. Non-sensitized red blood cells are also available in the kit. They ensure the specificity of the reaction by allowing identifying any interference from the natural anti-sheep agglutinins (Forssman heteroantibodies, infectious mononucleosis antibodies…). The reaction is carried out in a U-microplate. Each kit allows 120 tests to be carried out or 20 reactions of 6 dilutions. Results are obtained within 2 hours and are reliable overnight.


Simple protocol

A single protocol for all our ELI.H.A kits.

  1. First serum dilution
  2. Microplate preparation
  3. Serial dilution of serum and preparation of the ‘serum control’ well
  4. Addition of sensitized RBC and non sensitized RBC
  5. Gently mix the plate and let stand 2 hours before reading

eliha_methodology[2]

The following procedures are therefore valid for all our ELI.H.A range:


Easy-to-Read and Easy-to-Interpret Results

eli_ha_aspergillus[1]

Titer Interpretation
<1:320 Non-significant reaction
Probable absence of a deep aspergillosis infection. Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test.
=1:320 Doubtful reaction
Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test.
≥1:640 Significant reaction in favor of a deep aspergillosis infection

Reagents and Material

Quantity Description
1 R1: Vial of 2.2 mL of sensitized red blood cells
1 R2: Vial of 1mL of non-sensitized red blood cells
1 BUF: Vial of 55 mL of phosphate buffer pH 7.2
1 R3: Vial of 2 mL of adsorbent
1 CONTROL +: Vial of 0.2 mL of titrated positive control
1 CONTROL -: Vial of 0.2 mL of negative control
2 MICROPLATE: Microplate with a U-bottom
2 DROPPER: Special dropper

Material Required but Not Supplied

  • Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured
  • Contaminated waste containers
  • Centrifuge
  • Hemolysis tubes

Stability and Storage

  • The reagents are liquid and ready-to-use.
  • All the reagents are stored at 2-8°C. Do not freeze.
  • The results of the stability study indicate that the product has a stability of 24 months at 2-8°C from its manufacture.

Performances

Sensitivity Specificity
96.1% 96.6%

 

Manufacturer: ELITech Microbio.
Product(s) intended for healthcare professionals.
Read the instructions on the label and/or instructions for use of the product(s).

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