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PRODUCT
ELI.H.A Aspergillus
Indirect hemagglutination test for serodiagnosis of Aspergillosis.
ELI.H.A Aspergillus enables the quantitative determination, by indirect hemagglutination, of serum antibodies present in patients suffering from aspergillosis (Aspergillus fumigatus).
- Liquid ready-to-use reagents
- Cost-effective test
- High sensitivity and specificity
- Fast results for clinicians (2 hours)
- Simple visual reading, without expert interpretation
| Reference | Name | Quantity |
| 44602 | ELI.H.A Aspergillus | 102 tests |
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Benefits
Intended use
- Local conditions (preformed cavities, debrided pulmonary abscesses, impaired mucous membranes…);
- General conditions (immunosuppression following major surgery or medical procedures: organ transplantation, immunosuppressive therapy, steroids, antibiotics…).
ELITechGroup developed the ELI.H.A Aspergillus, an indirect hemagglutination assay, for the quantitative determination of anti-Aspergillus fumigatus antibodies in the serum. It is rapid, easy-to-use and easy-to-read with results available within 2 hours.
Principle
Sheep red blood cells are sensitized with Aspergillus fumigatus antigen. Diluted serum is mixed with sensitized sheep red blood cells. If anti-Aspergillus fumigatus antibodies are present in the serum, sensitized red blood cells will agglutinate, resulting in a cloudy red/brown deposit coating the well. In the absence of specific antibodies, sensitized red blood cells will not agglutinate, resulting in a ring-like deposit at the bottom of the well. Non-sensitized red blood cells are also available in the kit. They ensure the specificity of the reaction by allowing identifying any interference from the natural anti-sheep agglutinins (Forssman heteroantibodies, infectious mononucleosis antibodies…). The reaction is carried out in a U-microplate. Each kit allows 120 tests to be carried out or 20 reactions of 6 dilutions. Results are obtained within 2 hours and are reliable overnight.
Simple protocol
A single protocol for all our ELI.H.A kits.
- First serum dilution
- Microplate preparation
- Serial dilution of serum and preparation of the ‘serum control’ well
- Addition of sensitized RBC and non sensitized RBC
- Gently mix the plate and let stand 2 hours before reading
![eliha_methodology[2]](http://www.elitechgroup.com/wp-content/uploads/2015/01/eliha_methodology2.jpg)
The following procedures are therefore valid for all our ELI.H.A range:
Easy-to-Read and Easy-to-Interpret Results
![eli_ha_aspergillus[1]](http://www.elitechgroup.com/wp-content/uploads/2015/01/eli_ha_aspergillus1.jpg")
| Titer | Interpretation |
| <1:320 | Non-significant reaction Probable absence of a deep aspergillosis infection. Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test. |
| =1:320 | Doubtful reaction Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test. |
| ≥1:640 | Significant reaction in favor of a deep aspergillosis infection |
Reagents and Material
| Quantity | Description |
| 1 | R1: Vial of 2.2 mL of sensitized red blood cells |
| 1 | R2: Vial of 1mL of non-sensitized red blood cells |
| 1 | BUF: Vial of 55 mL of phosphate buffer pH 7.2 |
| 1 | R3: Vial of 2 mL of adsorbent |
| 1 | CONTROL +: Vial of 0.2 mL of titrated positive control |
| 1 | CONTROL -: Vial of 0.2 mL of negative control |
| 2 | MICROPLATE: Microplate with a U-bottom |
| 2 | DROPPER: Special dropper |
Material Required but Not Supplied
- Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured
- Contaminated waste containers
- Centrifuge
- Hemolysis tubes
Stability and Storage
- The reagents are liquid and ready-to-use.
- All the reagents are stored at 2-8°C. Do not freeze.
- The results of the stability study indicate that the product has a stability of 24 months at 2-8°C from its manufacture.
Performances
| Sensitivity | Specificity |
| 96.1% | 96.6% |
Manufacturer: ELITech Microbio.
Product(s) intended for healthcare professionals.
Read the instructions on the label and/or instructions for use of the product(s).
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