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PRODUCT
CANDIFAST®
Test combining identification and antifungal resistance testing of the main pathogenic yeasts.
Unique kit featuring a 2-in-1 technology: Identification and Resistance Testing from the same inoculum.
- Convenient: same inoculum and 20-well tray for the combined identification and resistance testing
- Cost-effective routine laboratory test
- High sensitivity and specificity
- Accurate results from isolated colonies usually available in 24 hours
- Simple colorimetric reaction, without expert interpretation
| Reference | Name | Quantity |
| 44008 / 44030 | CANDIFAST® | 8 / 30 tests |
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Benefits
Intended use
The frequency of fungal infections and in particular those associated with yeasts have increased considerably over the last ten years. The identification of the species, whether as a single agent or of different types, present in a clinical specimen has become indispensable for determining the correct therapeutic option. The CANDIFAST® kit allows the identification of the main human pathogenic yeasts as well as the testing of their resistance to various antifungal agents.
Procedure
Principle
The CANDIFAST® kit allows the identification of the following yeasts, Candida, Trichosporon, Cryptococcus,Rhodotorula, Saccharomyces genera. The main identification characteristics are: – Sensitivity to actidione – Fermentation of 7 carbohydrates – Hydrolysis of urea This test is combined with an antifungal resistant test adapted to the treatment of superficial mycosis : Amphotericine B, Nystatine, Flucytosine, Econazole, Ketoconazole, Miconazole, Fluconazole. The test is performed using colonies which should be young (24 to 48 hours old) and perfectly isolated on an agar medium in a Petri dish. All the necessary reagents are contained in the 20-well tray (1 test/tray) and no additional reagent is required to perform the test.
Easy-to-read and easy-to-interpret results
Results are visualized by a simple spontaneous colorimetric reaction, without instrument or expert interpretation. Identification table & chart are available for the interpretation of the results. Markings on the test-tray adhesive tape may also be used.
Reagents and material
| Reference | Quantity | Description |
| 44008 / 44030 | 10 / 35 | R1: Vial of 4 mL of buffered agar medium for dilution and identification. |
| 44009 / 44030 | 8 / 30 | R2: Vial of 2 mL of YNB (yeast nitrogen base) medium containing urea and for resistance testing, phenol red. |
| 44010 / 44030 | 1 / 1 | TC: Vial of 4 mL of barium sulphate solution for the control of inoculum standardization. |
| 44011 / 44030 | 8 / 30 | CANDIFAST® Tray: 20 well-tray individually packed in an aluminum sachet (each tray allows the testing of one sample: identification + resistance). |
Stability and storage
The kit and its contents when stored at 2-8 °C in their original state are stable until the expiry date indicated on the box.
Performance
The evaluation of the performance characteristics was performed on different yeasts:
| From collection strains | From clinical strains | |
| Identification: The parallel comparative study was carried out with the bioMérieux API 32C method. | 97.5% strains were correctly identified | |
| Identification: The parallel comparative study was carried out with the bioMérieux VITEK method. | 98% strains were correctly identified | |
| Resistance test: The parallel comparative study was carried out with the Biorad FUNGITEST and the bioMérieux ATB Fungus trays. | The overall agreement was 87.4% | The overall agreement was 98.5% |
Material required but not provided
- Paraffin oil
- Sterile pipettes
- Incubator at 37 °C
- Container for contaminated waste
Manufacturer: ELITech Microbio.
Product(s) intended for healthcare professionals.
Read the instructions on the label and/or instructions for use of the product(s).
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