PRODUCT

Bordetella ELITe MGB® Kit

Bordetella species are Gram-negative bacteria responsible for respiratory infections in humans. Among them, Bordetella pertussis is the primary causative agent of pertussis (whooping cough), while Bordetella parapertussis and Bordetella holmesii can cause pertussis-like respiratory disease. Pertussis typically begins with nonspecific respiratory symptoms and can progress to severe, persistent coughing episodes, sometimes accompanied by the characteristic inspiratory “whoop” cough and vomiting. The disease can affect individuals of all ages, but infants are at greatest risk of severe complications, including apnea, pneumonia and, in the most severe cases, death. Differentiating the causative Bordetella species can be challenging, as infections may present with overlapping clinical manifestations.

Molecular testing enables rapid and sensitive detection and differentiation of Bordetella species, supporting accurate diagnosis and appropriate patient management. Bordetella ELITe MGB® Kit is a qualitative multiplex Real-Time PCR assay for the detection and identification of the genomic DNA of Bordetella pertussis (BP), Bordetella parapertussis (BPP) and Bordetella holmesii (BH), extracted from clinical specimens.

The assay is validated for use with ELITe InGenius® and ELITe BeGenius® instruments for nasopharyngeal aspirate matrix.

Benefits

Target gene
  • IS481 repeated sequence (B. pertussis and B. holmesii)
    promoter of ptxA gene (B. pertussis)
    recA gene (B. holmesii)
    IS1001 repeated sequence (B. parapertussis)
Easy-to-use
  • Monoreagent, ready-to-use
Kit format
  • 96 tests (8 tubes x 12 tests each)
Analysis type
  • Qualitative
Validated matrices
  • Nasopharyngeal aspirate
Minimum sample volume required
  • 200 μl for all matrices
Sample-to-Result workflow
  • Fully automated process including extraction, amplification and result analysis with ELITe InGenius®, ELITe BeGenius®
Regulatory status
  • Whole process CE-IVD (IVDR) compliant
Trusted technology
  • ELITe MGB® probes designed with Minor Groove Binder technology, Superbases® and Eclipse® Dark Quencher
Wide panel
  • Comprehensive solution for the investigation of respiratory pathogen infections

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