Cell culture

Products

KPL/SeraCare Secondary antibodies

KPL secondary antibodies are ideal for use in immunoassay applications such as ELISA, immunoblotting and immunohistochemistry. With a broad portfolio of conjugate options and the use of affinity purification, KPL antibodies support the individual needs of researchers and IVD developers worldwide. To learn more about the performance of KPL antibodies in various types of immunoassays, view our Antibody Application Guide.

Affinity purification for optimal detection
Affinity purified antibodies are useful as primary and secondary antibodies in heterogeneous immunoassays and make excellent secondary anti-species (e.g., Goat anti-human IgG) antibody conjugates. Affinity purified antibodies are especially valuable in immunoassays where extraneous serum proteins and unknown antibodies can interfere with test results.

Diverse conjugate options for enhanced performance
KPL’s affinity purified polyclonal antibodies are available unlabeled or labeled with enzymes, biotin or a variety of fluorochromes. To search for your antibody of interest, simply utilize the product filter to the left, or view a detailed list of our antibody conjugate applications.

Amresco Biochemicals and Life science products

High quality biochemicals and reagents for Molecular Biology, Life Sciences, Proteomics, Clinical and Histology areas of research

Whether for applications in DNA, RNA, Protein, Cell Culture or more, AMRESCO products deliver solutions that provide quality, convenience and performance—so that researchers can focus on the future, transforming possibilities into reality.

AMRESCO is a manufacturer and supplier of high quality biochemicals and reagents for Molecular Biology, Life Sciences, Proteomics, Clinical and Histology areas of research. Founded in 1976, AMRESCO supplies standard and custom products to our customers in the following markets: Diagnostics, Molecular Biology, Biotechnology and Research Specialty Chemicals.

Major Product Groups

  • Agaroses & Acrylamides
  • Amino Acids
  • Antibiotics
  • Antimycotics
  • Biological Compounds
  • Buffers
  • Carbohydrates
  • Chaotrophic Agents
  • Chelating Agents
  • Detergents
  • Dyes/Stains
  • Enzymes
  • Media
  • Phenols/Organics
  • Preservative
  • Reagents
  • Reducing Agents
  • Salts
  • Substrates
  • Sugar

Axis-Shield Density Gradient Media

No other density gradient media conform to such strict clinical criteria.

Much of modern cell and molecular biology relies on the isolation of pure, functionally intact populations of cells, subcellular organelles and macromolecules in high yield and both research and clinical applications provide an impetus for the development of improved separation technology.

The most commonly used method of separating suspensions of mixed populations of biological particles is centrifugation in density gradients – and the choice of medium used to form the gradients is critical for the retention of particles function. Since the early sixties Nycomed have been developing and producing iodinated compounds as X-ray imaging agents. It was recognized early on that these compounds were also well suited to the formation of density gradients and offered new opportunities for improved methods of separating biological particles, especially osmotically-sensitive cells and subcellular organelles. Nycomed has therefore been avtively involved over the last 30 years in the research and development of density gradient media.

Recently, a new chapter opened in Nycomed’s history, when in 2000, Axis-Shield took over responsibility for these products. Today, Axis-Shield offers a range of iodinated density gradient media, produced in compliance with EU cGMP standards and regulatory requirements, which can be used for the purification not only of cells and organelles, but also membrane vesicles, viruses, proteins, nucleic acids and lipoproteins. The use of these compounds as contrast media demanded that they be rigorously tested to ensure their safety and suitability for clinical use. Axis-Shield density gradient media are therefore proven to be non-toxic; have a low osmolality and are sufficiently dense to permit the purification of all types of biological particles.

Detection, Prevention & Elimination of Contamination in Cell Cultures + PCR cycler validation

Minerva Biolabs GmbH is a leading biotechnology company for the development and marketing of detection & elimination kits for the control of microbial contamination. The company is based in Berlin. Their core competence lies in the control of mycoplasma, bacteria and viruses in cell cultures and biopharmaceuticals. In addition they employ their specialized kits and reagents as PCR diagnostics of respiratory tract infections and for the detection of legionella in water. They regard it as their function to be your highly competent expert in these specialized areas of contamination control, clinical diagnostics and water diagnostics.


The Mycoplasma Detection Kits employ PCR technology for rapid and reliable detection of more
than 110 Mollicutes species, including Mycoplasma, Acholeplasma and Spiroplasma. All relevant
species known as contaminants in various in situ biologicals including cell cultures, culture media, cell
preparations and virus stocks are detectable. One of the different types will fit each individual customer’s
need for a fast, reliable and time-saving routine monitoring!
Mynox® is a biological agent that integrates selectively into the mycoplasma membrane
and compromises its integrity. The result is an osmotic influx that leads to the complete
disintegration of the mycoplasma membrane. With mycoplasma eradicated, cells can
immediately return to their native morphology and normal proliferation rates.


Onar® Bacteria utilizes the polymerase chain reaction (PCR) for highly sensitive detection of
bacterial contamination in various in situ biologicals including cell cultures and virus stocks.
The primer set is specific to a highly conserved region within the 16S ribosomal RNA gene in
the bacteria genome. This allows for detection of all bacteria species tested thus far and usually
encountered as airborne contaminants in cell cultures. Eukaryotic DNA is not amplified by
Onar® Bacteria. The Primer/Nucleotide Reaction Mix already provides an Internal Control DNA as an
essential tool for successfully executed reactions.


ZellShield® protects cell cultures from a broad range of common contaminants.
ZellShield® is active against most intracellular and extracellular growing gram negative and gram
positive bacteria, mycoplasma, protozoon, fungi and yeast.
WaterShield™ protects the water in the reservoir from microbial growth. WaterShield™ can be used in
any kind of laboratory water reservoirs and is compatible with common work surfaces.
Mycoplasma-Off™ is a highly effective solution for cleansing and disinfection of all suitable laboratory
surfaces and apparatus. Mycoplasma-Off™ is outstandingly safe in use possessing not only a
strong mycoplasmacidal effect, but also being effective against bacteria, yeasts and viruses: Mycoplasma-
Off™ has been proven to be effective against bacteria such as Staphylococcus aureus,
Enterococcus hirae, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Candida albicans,
BVDV, Poliovirus, Adenovirus, Polyomavirus SV40, Norovirus and Vacciniavirus.


The PCR Cycler Check™ kits allow a realistic verification of block and air heated PCR cyclers for installation
qualification (IQ), operational qualification (OQ) and performance qualification (PQ) according
to legal requirements, like ISO 17025, EN 45001, ISO 13485, ISO/TS 20836:2007, GLP, GMP and
others.
The PCR Cycler Check™ series provides temperature sensitive PCR reactions to monitor an upper and
lower temperature range in one PCR reaction. The primer sequences and the PCR protocol were designed
to react extremely sensitive on fluctuations of more than 2 °C within the cycler protocol with a drop
out of amplification. This way the temperature homogeneity, the precision of the temperature control
and the timing can be verified. In addition, the pre-adjusted target concentrations are only amplified at
high PCR efficiencies as an additional indicator for accurate temperature control of the thermal cycler.


AquaScreen® is a test system for quantitative detection of water pathogens. AquaScreen® combines
water filtration, lysis of the collected microorganisms, DNA extraction and elution of the DNA in minimal
volumes ready for subsequent PCR analysis.


Food Control™ kit series includes Food Control™ qPCR, Food Control™ LFA and Food Control™ LFA+
PCR kits for fast and reliable detection of foodborne pathogens via real-time PCR or via our proprietary
Lateral Flow Assay (LFA) technology, respectively. Food Control™ qPCR is a diagnostic system for easy
determination of contamination degree in agricultural or food industry via real-time PCR. LFA technology
provides stripes as an easy read-out making DNA gels obsolete and in combination with our LFA+
cartridges and pre-cast chambers provide maximum contamination prevention. Food Control™ kits can
be combined with our optimized DNA extraction system ExtractNow™ Food Control.


We offer our highly effective decontamination products WaterShield™, Lab Clean™, PCR Clean™ and
PCR Clean™ Wipes in order to help you to protect your research. Watershield™ is a disinfection additive
for incubators and water baths and is active against a broad range of typical water contaminations from
bacteria, mycoplasma, protozoan, algae, fungi and yeast. Water exchange is conveniently indicated by
a diminishing blue stain. Lab Clean™ is a large area decontamination solution useful for floors preventing
room-to-room spreading of e.g. DNA carry over or trace amounts of DNA, keeping your lab “DNAload”
low if regularly applied. Finally, protect your PCR experiments from e.g. carry over or DNases and
RNases using PCR Clean™ or PCR Clean™ Wipes highly effective surface decontamination reagents.

KPL TrueBlue Peroxidase Substrate

TrueBlue is up to 100 times more sensitive than DAB

TrueBlue is up to 100 times more sensitive than DAB and it is free of known carcinogens. Strong blue reaction product provides excellent contrast with DAB and other substrates. HistoMark TrueBlue provides the most sensitive means available to detect peroxidase-labeled conjugates. Because of its high sensitivity, TrueBlue is recommended for detection of the least abundant marker. TrueBlue is alcohol and xylene insoluble.