Indirect hemagglutination test for serodiagnosis of amoebiasis.
ELI.H.A Amoeba enables the quantitative determination, by indirect hemagglutination, of serum antibodies present in patients suffering from amoebiasis (Entamoeba histolytica).
- Cost-effective test
- Easy and ready-to-use
- High sensitivity and specificity
- Fast results for clinicians (2 hours)
- Simple visual reading, without expert interpretation
Product Description: ELI.H.A Amoeba
Number of Tests: 120
Amoebiasis is a parasitic infection caused by a protozoon specific to humans: Entamoeba histolytica. It’s the only pathogenic amoeba for humans. Immunological diagnosis, via the detection of antibodies, is highly important to confirm hepatic and pulmonary amoeba infections.
ELITech developed the ELI.H.A Amoeba, an indirect hemagglutination assay, for the quantitative determination of anti-Entamoeba histolytica antibodies in the serum. It is rapid, easy-to-use and easy-to-read with results available within 2 hours and reliable overnight.
Sheep red blood cells are sensitized with Entamoeba histolytica antigen.
Diluted serum is mixed with sensitized sheep red blood cells. If anti-Entamoeba histolytica antibodies are present in the serum, sensitized red blood cells will agglutinate, resulting in a cloudy red/brown deposit coating the well. In the absence of specific antibodies, sensitized red blood cells will not agglutinate, resulting in a ring-like deposit at the bottom of the well.
Non-sensitized red blood cells are also available in the kit. They ensure the specificity of the reaction by allowing identifying any interference from the natural anti-sheep agglutinins (Forssman heteroantibodies, infectious mononucleosis antibodies…).
The reaction is carried out in a U-microplate.
Each kit allows 120 tests to be carried out or 20 reactions of 6 dilutions. Results are obtained within 2 hours and are reliable overnight.
A single protocol for all our ELI.H.A kits.
1. First serum dilution
2. Microplate preparation
3. Serial dilution of serum and preparation of the ‘serum control’ well
4. Addition of sensitized RBC and non sensitized RBC
5. Gently mix the plate and let stand 2 hours before reading
Easy-to-read and easy-to-interpret results
Probable absence of amoeba tissue infection
A control must be carried out with the electrosyneresis technique, latex agglutination technique (ELITex Bicolor Amoeba) and indirect immunofluorescence.
|≥1:320||SIGNIFICANT REACTION IN FAVOR OF VISCERAL AMOEBIASIS|
Reagents and Material
Vial of 2.4 mL of sensitized red blood cells
Vial of 1 mL of non-sensitized red blood cells
Vial of 55 mL of phosphate buffer pH 7.2
Vial of 2 mL of adsorbent
- CONTROL +:
Vial of 0.2 mL of titrated positive control
- CONTROL -:
Vial of 0.2 mL of negative control
Microplate with a U-bottom
Stability and Storage
- The reagents are ready-to-use.
- All the reagents are stored at 2-8°C. Do not freeze.
- The results of the stability study indicate that the product has a stability of 24 months from its manufacture.
- Diagnostic sensitivity: 93%
- Diagnostic specificity: 97%
Material required but not provided
- Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured
- Contaminated waste containers
- Hemolysis tubes