CTR-CPE is a universal Internal Control suitable for all ELITe MGB & Alert Q-PCR kits. The Internal Control is validated for DNA and RNA extraction from non-cellular and cellular biological samples with the ELITechGroup products: EXTRAgen, EXTRAzol, EXTRAblood, ELITe STAR 200 Extraction kit, ELITe GALAXY 300 Extraction Kit, ELITe InGenius SP200 and also with NucliSENS® easyMAG® Reagents (bioMérieux), QIAsymphony® DSP Virus / Pathogen Midi kit and QIAsymphony® DNA Mini kit (QIAGEN).
CTR-CPE is a stabilised plasmid solution containing two plasmid DNAs and genomic RNA of MS2 phage, aliquoted in four tubes and ready for use.
The amplification of the internal control CTR-CPE indicates the correctness of the extraction procedure: the presence of DNA/RNA and the absence of inhibitors.
ELITechGroup Molecular Diagnostics offers systems for the detection and identification of DNA (derived from nucleic acid amplification) by electrophoretic separation on agarose gel. The product intended use is to detect the DNA amplicons obtained by qualitative nucleic acids amplification assays.
The kit provides ready to use agarose gels in TAE buffer 1x with ethidium bromide, an electrophoresis buffer 50x to be reconstituted with sterile bidistilled water, a loading buffer 6x to prepare the amplification reaction, a ready to use molecular weight standard and a solution of ethidium bromide to dye the gel.
The RT – kit plus is a reverse transcription reagent for the synthesis of cDNA from RNA extracted from cellular and non-cellular samples.
The synthesized cDNA is used for the detection and quantification of human RNA viruses and human messenger RNA with diagnostic assays based on nucleic acids amplification with DNA polymerase enzymes (such as thermostable DNA polymerases).
The procedure consists of an enzymatic reaction with reverse transcriptase in a programmable thermal cycler. The reaction uses random hexamer primers and the RNA dependent DNA polymerase from the Moloney Murine Leukaemia Virus (MMLV). The reaction generates cDNA from all the different RNA molecules extracted from the samples. The “RNasi STOP” is an inhibitor that prevents extracted RNA from degradation in case of RNases contamination.
The efficiency of the reaction allows obtaining a quantity of cDNA, detectable using an amplification assay that uses 10 μl of the product of the reverse transcription product, starting from approximately 25 target RNA molecules in 10 μl of RNA extract added to the “monotest” test tube.
ELITech Molecular Diagnostic offers a range of nested PCR kits for diagnosis of some leukemia, lymphoma and melanoma. These products are an aid in the cancer diagnosis, alongside patient clinical data and other laboratory test outcomes.
The oligomix Alert kits are based on nested PCR technology (two consecutive amplification reactions). After the amplification, the presence of specific amplification products is detected by electrophoresis migration on agarose gel.