Detection, identification, and enumeration of urogenital mycoplasma in one simple test.
MYCOFAST® US has been designed for the detection, identification, and enumeration of Ureaplasma urealiticum (U.u.) and Mycoplasma hominis (M.h.) in endocervical, urethral, urinary, gastric,and sperm specimens.
- All-in-one test
- Ready-to-use reagents, “all-liquid” method
- High sensitivity and specificity
- Fast results for clinicians, available within 24 hours
- Visual colorimetric method, without expert interpretation
Featuring ready-to-use reagents and an “all liquid” method, MYCOFAST® US identifies the growth of U.u. and M.h. after 24 hours of incubation. Our exclusive growth activator, the M.h. Supplement, guarantees fast and accurate results.
MYCOFAST® US enables the enumeration of U.u. > 103 UCC/mL which is compliant with pathological thresholds recognized for sperm and urine samples.
Product Description: MYCOFAST® US
Number of Tests: 25 tests
Mycoplasmas are the smallest and simplest of the procaryotypes capable of self-reproduction (0.15 to 0.25 μm). They differ from other bacteria in their lack of a cell wall and hence a natural resistance to ß-lactams. Since mycoplasmas are relatively fragile, they will only grow in acellular cultures in the presence of various growth factors and at a constant temperature of 35 to 37 °C.
Most human mycoplasmas are commensal. Of the 9 species that have been isolated from the urogenital tract, Ureaplasma urealyticum and Mycoplasma hominis are the most commonly found. U.u. and M.h. are sexually transmitted and can be pathogenic. Respiratory infections or meningitis can occur in the neonate as a result of contamination from the genital tract at birth. In adults, the infections caused by U.u. and M.h. are described in the table below:
Conventional diagnosis is based upon culture on A7 agar plates followed by microscopical identification of U.u. (sea urchin shaped) or M.h. (fried-egg shaped) colonies.
Since both U.u. and M.h. are commensal, infection can only be diagnosed through the determination of the pathological threshold, followed by precise enumeration.
MYCOFAST® US gives the solution and makes your urogenital diagnosis much easier.
MYCOFAST® US identifies U.u. and M.h. growth after a 24 hour incubation in a liquid medium. During growth, U.u. and M.h. metabolize urea and arginine respectively resulting in a color change of the medium, which contains phenol red indicator, from yellow-orange to red. This color change is due to liberation of ammonia resulting in an alkaline pH of the medium.
Mycoplasma growth thus viewed enables:
- Enumeration of mycoplasma based on the rate of urea or arginine hydrolysis, which is proportional to the number of germs contained in the sample.
U.u: > 103, 104 and 105 UCC/ml
M.h: > 104 UCC/ml
- Identification based on the sensitivity or otherwise of the germ to three antibiotics (lincomycin, SXT and erythromycin) = Identibiotic.
Easy-to-read and easy-to-interpret results
The results are read by the color obtained in the different wells. Urogenital mycoplasma growth is indicated when the medium turns red (alkaline). The medium remains yellow when no growth of urogenital mycoplasma occurs.
Reagents and Material
Vial of mycoplasma transport medium (2 mL)
Vial of lyophilized growth medium (+2 mL UMMt)
- MYCOFAST® US trays:
Tray of 2 X 10-wells sealed
Mycoplama hominis growth activator (4.5 mL)
Material required but not provided
- Sample collecting material (Swabs, cytobrushes, sterile containers for liquid samples)
- Pipettes and tips
- Waste container for contaminated waste
- Paraffin oil
- Incubator at 37 ± 1 °C