Transplant Pathogen Monitoring

Parvovirus B19 ELITe MGB® Kit

Parvovirus B19 is a highly infectious and prevalent virus in the general population, acquired mostly during childhood, and responsible for a skin rash illness called “fifth disease”. Latent Parvovirus B19 can be reactivated after kidney or hematopoietic stem cell transplantation, and generates serious complications, especially in anemic patients, by impairing red blood cell production. Parvovirus B19 detection and viral load monitoring is crucial among these patients in order to initiate early polyvalent immunoglobulins treatment and prevent transplant failure.

Parvovirus B19 detection and quantification is also important during pregnancy to confirm an ongoing infection when foetal defects are observed during ultrasound scan, and initiate the appropriate therapy method early.

Parvovirus B19 ELITe MGB® Kit is a real-time PCR assay designed for the detection and quantification of Parvovirus B19 DNA. The assay is CE-IVD validated on different sample types, in combination with ELITe InGenius®, a fully automated sample-to-result solution.

Benefits

Conserved targets · Genomic region of parvovirus B19 coat protein VP1 and human beta-globin gene as an internal control
Easy-to-use · Monoreagent, ready-to-use
Best-in class PCR technology · ELITe MGB probes designed with Minor Groove Binder, Superbases® and Eclipse® Dark Quencher
Samples · Whole blood and amniotic fluid
Integrated workflow · Fully automated process for extraction, amplification and result analysis with ELITe InGenius
High performance · 100% clinical sensitivity and 100% clinical specificity
Quantitative result · International units (IU/mL) correlated to the 2nd WHO International standard for Parvovirus B19 DNA for NAAT (NIBSC Code 99/802)
· Copies/mL (cp/mL)
Storage of standard curve · 60 days
Eluate storage · Additional testing including multiple PCR from the same eluate, retesting or archiving
Minimal hands-on time · Less than 2 minutes per sample from extraction to result analysis
Complete transplant menu, pregnant women testing · Complete transplant pathogen monitoring range
· Pregnant women testing with CMV ELITe MGB kit* and Toxoplasma g. ELITe MGB kit CE-IVD validated for amniotic fluid

*CE-IVD validation ongoing

 

Ordering Information

Product Name Description Reference
Parvovirus B19 ELITe MGB Kit 4 tubes, 24 reactions each RTS070PLD
Parvovirus B19 ELITe Standard 2 sets of 4 levels, 8 reactions each STD070PLD
Parvovirus B19 ELITe Positive Control 2 tubes, 4 reactions each CTR070PLD
CTRCPE – Internal Control 4 tubes, 12 reactions each CTRCPE

 

Parvovirus B19 ELITe MGB® Kit is also CE-IVD validated with other systems