Transplant Pathogen Monitoring

JCV ELITe MGB® Kit

John Cunningham Virus (JCV) is a widespread, asymptomatic polyomavirus sharing high homology with BK virus and establishing latency in the kidney. JCV reactivation, after hematopoietic stem cell or solid organ transplantation, is associated with numerous infections of the central nervous system. JCV viral load testing is important to monitor and prevent such complications in transplant patients.

JCV ELITe MGB® Kit is a real-time PCR assay designed for the detection and quantification of JC polyomavirus DNA. The assay is CE-IVD validated on a large range of sample types, in combination with ELITe InGenius®, a fully automated sample-to-result solution.

Benefits

Conserved targets · Region of John Cunningham Virus large T antigen gene and an artificial DNA sequence as an internal control
Easy-to-use · Monoreagent, ready-to-use
Best-in class PCR technology · ELITe MGB probes designed with Minor Groove Binder, Superbases® and Eclipse® Dark Quencher
Large range of samples · Plasma, urine and cerebrospinal fluid
Integrated workflow · Fully automated process for extraction, amplification and result analysis with ELITe InGenius
High performance · 98.7% clinical sensitivity and 98.6% clinical specificity
Quantitative result · International units (IU/mL) correlated to the 1st WHO International standard for Human JCV for NAAT (NIBSC Code 14/114)
· Copies/mL (cp/mL)
Storage of standard curve · 60 days
Eluate storage · Additional testing including multiple PCR from the same eluate, retesting or archiving
Minimal hands-on time · Less than 2 minutes per sample from extraction to result analysis
Complete transplant menu · Complete transplant pathogen monitoring range

 

Ordering Information

Product Name Description Reference
JCV ELITe MGB Kit 4 tubes, 24 reactions each RTS176PLD
JCV ELITe Standard 2 sets of 4 levels, 8 reactions each STD176PLD
JCV ELITe Positive Control 1 tube, 5 reactions CTR176PLD
CTRCPE – Internal Control 4 tubes, 12 reactions each CTRCPE

 

JCV ELITe MGB Kit is also CE-IVD validated with other systems.