ELITechGroup proprietary technologies

Unique Real-Time PCR technologies

Over the years, we have developed a comprehensive suite of PCR technologies, including the revolutionary Minor Groove Binder (MGB), to design superior sensitivity and specificity real-time PCR assays beneficial for laboratories, and clinicians.

 

Pleiades probes
Pleiades probes are proprietary probes concentrating all ELITechGroup PCR technologies, designed with a Minor Groove Binder (MGB), Eclipse® Dark Quencher, Aquaphluor® dyes, and Superbases®
• Very low background and high hybridization-triggered fluorescence
• Unique quenching effects with the combined action of MGB and Eclipse® Dark quencher
• Exceptional specificity due to a shorter length
• Improve sensitivity by combination high hybridization signal with low and stable background
• Melt-curve analysis capability

Probe

MGB 

MGB is a synthetic molecule attached to the 5’-end of MGB DNA probes, that binds in the minor groove of hybrid DNA molecules
• Increase stability of hybrid DNA enabling the design of shorter probes for excellent specificity and sensitivity polymorphisms discrimination
• Identify emerging mutations and reliably discriminate clinically important variants
• Increase reproducibly in A-T and G-C rich regions
• Enable reliable, clear and unambiguous melt-curve analysis
Eclipse® DarkQuencher
Eclipse® DarkQuencher absorbs the fluorophore energy and dissipates it as heat; while a typical quencher re-emits part of the energy as light
• Improve absorbance of fluorescence emitted from dye reagents
• Decrease background level of fluorescence
• Improve PCR assay sensitivity
• Cover 4 spectra wavelengths
Aquaphluor® dyes
Aquaphluor® dyes are fluorescent compounds that can re-emit light upon light excitation
• Comprehensive range of 25 different colours from UV to far-red developed
• Perfectly adapted to multiplexed assays
Superbases: Super A, Super T, Super G, and Super I 

Superbases are engineered nucleotides selectively used to optimize primers and probes design
• Enhance A-T stability (e.g. rich A/T domains)
• Reduces secondary structure (e.g. GG self-association) and fluorescence quenching
• Confer close Tm value when paired with any bases