Onco-hematology

BCR-ABL P210 ELITe MGB® Kit

The product of the Philadelphia translocation, the BCR/ABL oncogene P210 isoform, is typically found in chronic myelogenous leukemia, and in acute lymphoid and myeloid leukemia, causing the cells to divide uncontrollably and inducing severe outcomes among patients. Detection and quantification of P210 BCR-ABL fusion gene, by molecular methods, is crucial for patient management, to initiate the appropriate treatment, measure the response to therapy and predict relapse

 

BCR-ABL P210 ELITe MGB® Kit is a real-time PCR assay for the quantification of the mRNA of the BCR-ABL rearrangement, t(9;22) translocation of the Philadelphia chromosome, isoform P210 compared to the mRNA of the gene coding the kinase protein Abelson (ABL) mRNA. The assay has been tested on a large range of sample types with conventional extraction and PCR systems.

Benefits

Conserved targets · Fusion gene and BCR-ABL, isoform P210
· Human gene ABL
One-step format · Reverse transcription and real-time PCR assay within the same run
Best-in class PCR technology · ELITe MGB probes designed with Minor Groove Binder, Superbases® and Eclipse® Dark Quencher
Large range of samples · Peripheral blood samples, medullary blood samples collected in citrate or EDTA, lymphomonocyte or leukocyte suspensions
Instruments · Extraction: TRI reagent product (Thermo Fisher Scientific) and Maxwell 16 (Promega)
· Thermal cyclers: ABI 7300 Real-Time PCR system and ABI 7500 series
High performance · 100% clinical sensitivity and 100% clinical specificity
Complete Onco-hematology menu · Complete Onco-hematology menu with BCR-ABL P190 ELITe MGB® Kit & Philadelphia P210 RNA*

*The Philadelphia P210 RNA reference is a reference material calibrated against the “1st world health organization (WHO) international Genetic Reference Panel for quantification of BCR-ABL translocation by RQ-PCR” enabling to determine a conversion factor to correlate the results obtained with BCR-ABL P210 ELITe MGB® Kit to the International Scale.

Ordering Information

Product Name Description Reference
BCR-ABL P210 ELITe MGB Kit P210 PreMix:1 tube, 50 reactions
ABL PreMix: 1 tube, 50 reactions
PCR MasterMix: 2 tubes, 50 reactions each
RT EnzymeMix: 2 tubes,50 reactions each
RTSG07PLD210
BCR-ABL P210 ELITe Standard 2 sets of 4 levels, 2 reactions each STDG07PLD210
Philadelphia P210 RNA Reference 1 set of 4 levels, 4 reactions SPG07-210