Related Products

Products

Q-PCR Alert Kits – Microbiology

TaqMan® Real-Time PCR
ELITech Molecular Diagnostic offers a wide range of Real-Time PCR kits for treatment and monitoring of opportunistic infections and infectious diseases. TaqMan® is a registered trademark of Roche Molecular Systems, Inc.

These products are based on TaqMan®-MGB technology and feature:

  • MGB (Minor Groove Binder) molecules to increase specificity
  • UNG (Uracil-N glycosidase) to prevent false positive results
  • Passive reference dye to calibrate the basic fluorescence
  • Internal control to avoid false negative results

All the TaqMan® based assays have a common temperature amplification profile allowing multi target runs on the same instrument at the same time to streamline operations and enhance daily workflow.

Q-PCR Alert Kits – Onco-hematology

TaqMan® Real-Time PCR

The Philadelphia Q – PCR Alert Kits are quantitative Real-Time reverse-transcription PCR assay for detection of the BCR-ABL rearrangement variants p210 or p190 (also known as t(9;22) translocation, Philadelphia chromosome). They are also used for the quantification of the ratio between BCR-ABL and ABL gene. The products are intended for use in the diagnosis and follow up of chronic myelogenous leukemia (CML), alongside patient clinical data and other laboratory test outcomes. The products follow the ELN guidelines (European Leukemia Net website: www.leukemianet.org/content/home) for the management of CML patients from diagnosis to monitoring of minimal residual disease.

These products are based on TaqMan® technology and feature:

  • UNG (Uracil-N glycosidase) to prevent false positive results
  • Passive reference dye to calibrate the basic fluorescence
  • Internal control to avoid false negative results

All the TaqMan® based assays have a common temperature amplification profile allowing multi target runs on the same instrument at the same time to streamline operations and enhance daily workflow.

TaqMan® is a registered trademark of Roche Molecular Systems, Inc.

Q-PCR Alert Kits – Genetics

TaqMan® Real-Time PCR

ELITech Molecular Diagnostic offers a range of Real-Time PCR kits for allelic determination of thrombotic single nucleotide polymorphisms. These products are an aid in the thrombophilia diagnosis, alongside patient clinical data and other laboratory test outcomes.

These products are based on TaqMan®-MGB technology and feature:

  • MGB (Minor Groove Binder) molecules to increase specificity
  • UNG (Uracil-N glycosidase) to prevent false positive results
  • Passive reference dye to calibrate the basic fluorescence
  • Internal control to avoid false negative results

All the TaqMan® based assays have a common temperature amplification profile allowing multi target runs on the same instrument at the same time to streamline operations and enhance daily workflow.

TaqMan® is a registered trademark of Roche Molecular Systems, Inc.

Oligomix Alert Kits – Oncohematology

Nested PCR

ELITech Molecular Diagnostic offers a range of nested PCR kits for diagnosis of some leukemia, lymphoma and melanoma. These products are an aid in the cancer diagnosis, alongside patient clinical data and other laboratory test outcomes.

The oligomix Alert kits are based on nested PCR technology (two consecutive amplification reactions). After the amplification, the presence of specific amplification products is detected by electrophoresis migration on agarose gel.

Internal Control

CTR-CPE is a universal Internal Control suitable for all ELITe MGB & Alert Q-PCR kits. The Internal Control is validated for DNA and RNA extraction from non-cellular and cellular biological samples with the ELITechGroup products: EXTRAgen, EXTRAzol, EXTRAblood, ELITe STAR 200 Extraction kit, ELITe GALAXY 300 Extraction Kit, ELITe InGenius SP200 and also with NucliSENS® easyMAG® Reagents (bioMérieux), QIAsymphony® DSP Virus / Pathogen Midi kit and QIAsymphony® DNA Mini kit (QIAGEN).

CTR-CPE is a stabilised plasmid solution containing two plasmid DNAs and genomic RNA of MS2 phage, aliquoted in four tubes and ready for use.

The amplification of the internal control CTR-CPE indicates the correctness of the extraction procedure: the presence of DNA/RNA and the absence of inhibitors.

CE-IVD product

Electrophoresis

ELITechGroup Molecular Diagnostics offers systems for the detection and identification of DNA (derived from nucleic acid amplification) by electrophoretic separation on agarose gel. The product intended use is to detect the DNA amplicons obtained by qualitative nucleic acids amplification assays.

The kit provides ready to use agarose gels in TAE buffer 1x with ethidium bromide, an electrophoresis buffer 50x to be reconstituted with sterile bidistilled water, a loading buffer 6x to prepare the amplification reaction, a ready to use molecular weight standard and a solution of ethidium bromide to dye the gel.

Reverse Transcription

The RT – kit plus is a reverse transcription reagent for the synthesis of cDNA from RNA extracted from cellular and non-cellular samples.

The synthesized cDNA is used for the detection and quantification of human RNA viruses and human messenger RNA with diagnostic assays based on nucleic acids amplification with DNA polymerase enzymes (such as thermostable DNA polymerases).

The procedure consists of an enzymatic reaction with reverse transcriptase in a programmable thermal cycler. The reaction uses random hexamer primers and the RNA dependent DNA polymerase from the Moloney Murine Leukaemia Virus (MMLV). The reaction generates cDNA from all the different RNA molecules extracted from the samples. The “RNasi STOP” is an inhibitor that prevents extracted RNA from degradation in case of RNases contamination.

The efficiency of the reaction allows obtaining a quantity of cDNA, detectable using an amplification assay that uses 10 μl of the product of the reverse transcription product, starting from approximately 25 target RNA molecules in 10 μl of RNA extract added to the “monotest” test tube.