Open Chemistry Reagents

Stable-liquid and ready-to-use ELITechGroup Clinical Systems features a comprehensive menu of ready-to-use, liquid-stable reagents. Over 100 available assays with flexible reagent packaging and methods referenced to industry standards. ELITech is committed to ongoing development of new methods to provide a growing line of superior chemistry test menu. Applications are available for a variety of automated analyzers.

Products

Diazyme Total Bile Acids

The Diazyme Total Bile Acids Assay is a liquid stable system, ready to use for both manual methods and is adaptable for many automated chemistry analyzers. The assay has excellent sensitivity with a linear range from 0 – 180 μM. Diazyme’s unique enzyme cycling method amplifies signal for accurate and fast measurement using a small sample up to 50% less than conventional NBT methods making it an excellent choice. The assay has excellent precision with Intra-Assay Precision CV% of <4% and Inter-Assay Precision CV% of <3%. The following substances normally present in serum produced less than 10% deviation at the listed concentrations: Triglycerides at 750 mg/dL, Ascorbic acid at 50 mg/dL, Bilirubin at 50 mg/dL and Hemoglobin at 500 mg/dL. Packaged for optimal operator convenience reagent transfer can be eliminated for most chemistry systems with instrument specific packaging options including RocheTM Hitachi 917 series, Beckman AU (400/600/640/680), Beckman Synchron CX, and DXC.

Assay Principle
The reagents of the assay kit are in a stable liquid formulation that allows for ease of use coupled with enhanced performance characteristics. In the presence of Thio-NAD, the enzyme 3-α-hydroxysteroid dehydrogenase (3- α-HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3-α-HSD can convert 3-keto steroids and Thio- NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405 nm.

Intended use
Diazyme Total Bile Acids Assay Kit is intended for the in vitro quantitative determination of total bile acids (TBA) in human serum. Total bile acids are metabolized in the liver and serve as a marker for normal and abnormal liver function. Serum total bile acids are increased in patients with liver disease.

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Market Segment:

Open Chemistry Reagents

Stable-liquid and ready-to-use
ELITechGroup Clinical Systems features a comprehensive menu of ready-to-use, liquid-stable reagents. Over 100 available assays with flexible reagent packaging and methods referenced to industry standards. ELITech is committed to ongoing development of new methods to provide a growing line of superior chemistry test menu.

Applications are available for a variety of automated analyzers.

Enzymes

  • Alanine Aminotransferase (ALT/GPT)
  • Alkaline Phosphatase (ALP)
  • Amylase
  • Aspartate Aminotransferase (AST/GOT)
  • Creatine Kinase MB (CK-MB)
  • Creatine Kinase NAC (CK-NAC)
  • Gamma Glutamyl transpeptidase Plus (GGT Plus)
  • Lactate Dehydrogenase (LDH-L)
  • Lactate Dehydrogenase (LDH-P)

Substrates

  • Albumin
  • Bilirubin Direct
  • Bilirubin Total
  • Cholesterol
  • Cholesterol HDL
  • Cholesterol LDL
  • Creatinine
  • Glucose
  • Microprotein Plus
  • Total Protein
  • Total Protein Plus
  • Triglycerides
  • Urea UV
  • Uric Acid

Specific proteins

  • C-reactive Protein (CRP IP)
  • Haptoglobin
  • Glycosylated Hemoglobin (HbA1c)
  • Immunoglobin A (IgA IP)
  • Immunoglobin G (IgG IP)
  • Immunoglobin M (IgM IP)
  • Microalbumin
  • Orosomucoid
  • Prealbumin
  • Transferrin

Electrolytes and Ion Selective Electrodes (ISE)

  • Calcium Arsenazo
  • Chloride
  • Iron
  • Magnesium
  • Phosphorus
  • Carbon Dioxide (ISE)
  • Chloride (ISE)
  • Potassium (ISE)
  • Sodium (ISE

Freeze Dried or Powder

To deliver more flexible and cost effective solutions ECS also provide some assays which can be constituted to liquid to be used on the chemistry analyzer.

Enzymes

  • Cholinesterase
  • Lactate

Electrolytes

  • Iron TIBC

Open Chemistry Reagents

Stable-liquid and ready-to-use
ELITechGroup Clinical Systems features a comprehensive menu of ready-to-use, liquid-stable reagents. Over 100 available assays with flexible reagent packaging and methods referenced to industry standards. ELITech is committed to ongoing development of new methods to provide a growing line of superior chemistry test menu.

Applications are available for a variety of automated analyzers.

Enzymes

  • Alanine Aminotransferase (ALT/GPT)
  • Alkaline Phosphatase (ALP)
  • Amylase
  • Aspartate Aminotransferase (AST/GOT)
  • Creatine Kinase – MB (CK-MB)
  • Creatine Kinase (CKNAC)
  • γ – Glutamyltransferase (γ-GT)
  • Lactate Dehydrogenase (LDH)
  • Cholinesterase

Substrates

  • Albumin
  • Bilirubin Direct
  • Bilirubin Total
  • Bilirubin Direct & Total
  • Cholesterol
  • Cholesterol HDL
  • Cholesterol HDL Direct
  • Cholesterol LDL Direct
  • Creatinine
  • Lactate
  • Glucose
  • Hemoglobin
  • Protein Total (Serum)
  • Protein Total (Urine)
  • Triglycerides
  • Urea
  • Uric Acid

Specific proteins

  • C-reactive Protein (CRP IP)
  • Haptoglobin
  • Glycosylated Hemoglobin (HbA1c)
  • Immunoglobin A (IgA IP)
  • Immunoglobin G (IgG IP)
  • Immunoglobin M (IgM IP)
  • Microalbumin
  • Orosomucoid
  • Prealbumin
  • Transferrin

Electrolytes and Ion Selective Electrodes (ISE)

  • Calcium
  • Chloride
  • Iron
  • Magnesium
  • Phosphorus
  • Carbon Dioxide (ISE)
  • Chloride (ISE)
  • Potassium (ISE)
  • Sodium (ISE)

 

Open Chemistry Reagents

Stable-liquid and ready-to-use
ELITechGroup Clinical Systems features a comprehensive menu of ready-to-use, liquid-stable reagents. Over 100 available assays with flexible reagent packaging and methods referenced to industry standards. ELITech is committed to ongoing development of new methods to provide a growing line of superior chemistry test menu.

Applications are available for a variety of automated analyzers.

Enzymes

  • Alanine Aminotransferase (ALT/GPT)
  • Alkaline Phosphatase (ALP)
  • Amylase
  • Aspartate Aminotransferase (AST/GOT)
  • Creatine Kinase MB (CK-MB)
  • Creatine Kinase NAC (CK-NAC)
  • Gamma Glutamyl transpeptidase Plus (GGT Plus)
  • Lactate Dehydrogenase (LDH-L)
  • Lactate Dehydrogenase (LDH-P)

Substrates

  • Albumin
  • Bilirubin Direct
  • Bilirubin Total
  • Cholesterol
  • Cholesterol HDL
  • Cholesterol LDL
  • Creatinine
  • Glucose
  • Microprotein Plus
  • Total Protein
  • Total Protein Plus
  • Triglycerides
  • Urea UV
  • Uric Acid

Specific proteins

  • C-reactive Protein (CRP IP)
  • Haptoglobin
  • Glycosylated Hemoglobin (HbA1c)
  • Immunoglobin A (IgA IP)
  • Immunoglobin G (IgG IP)
  • Immunoglobin M (IgM IP)
  • Microalbumin
  • Orosomucoid
  • Prealbumin
  • Transferrin

Electrolytes and Ion Selective Electrodes (ISE)

  • Calcium Arsenazo
  • Chloride
  • Iron
  • Magnesium
  • Phosphorus
  • Carbon Dioxide (ISE)
  • Chloride (ISE)
  • Potassium (ISE)
  • Sodium (ISE

Freeze Dried or Powder

To deliver more flexible and cost effective solutions ECS also provide some assays which can be constituted to liquid to be used on the chemistry analyzer.

Enzymes

  • Cholinesterase
  • Lactate

Electrolytes

  • Iron TIBC