Bacteriology

Our Bacteriology range relies on multiple techniques: agglutination tests, specific culture media, but also trays for identification of germs or antimicrobial susceptibility testing. ELITechGroup is the world leader in urogenital mycoplasmas diagnosis. Discover our complete offer enabling culture on agar plates, serological diagnosis, numeration, identification and determination of resistance profiles to antibiotics.
 
Our Microbiology range also features products in other areas: Serological diagnosis of streptococcus A, the rapid identification of Staphylococcus aureus, the antimicrobial susceptibility testing of enterobacteriaceae in urinary tract infections, the complete identification of corynebacteriaceae and related species.

Products

Liofilchem MIC Test Strips

The Liofilchem® MIC Test Strips is a quantitative assay for determining the Minimum Inhibitory Concentration (MIC) of antimicrobial agents against microorganisms to indicate appropriate patient treatment and for identifying resistance patterns.
Liofilchem® MIC Test Strips are made of special high quality paper impregnated with a predefined concentration gradient of antibiotic, across 15 two-fold dilutions like those of a conventional MIC method.
On one side of the strip a MIC scale in µg/mL is displayed and a code that identifies the antimicrobial agent. Liofilchem® MIC Test Strips are available in a large variety of antibiotic configurations. Each configuration is available in blister packs of 10 strips, 30 strips and 100 strips.

 

METHOD PRINCIPLE
When the Liofilchem® MIC Test Strip is applied onto an inoculated agar surface, the preformed exponential gradient of antimicrobial agent is transferred into the agar matrix. After 18 hours incubation or longer, a symmetrical inhibition ellipse centered along the strip is formed. The MIC is read directly from the scale in terms of µg/mL, at the point where the edge of the inhibition ellipse intersects with the MIC Test Strip.
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Mast Antibiotic susceptibility test discs

Disc diffusion is still the most popular method for determining bacterial antibiotic susceptibility in the treatment of infectious disease, as highlighted by the variety of international standardised procedures eg.EUCAST( Europe), CLSI (USA), BSAC (UK), AFFSAPS (France), DIN (Germany) & ISC/WHO.

mastdiscsTM AST are available as an extensive range of antibiotic susceptibility test discs presented in vials, or plastic dispensing cartridges.

Manufactured and QC tested in accordance with the accepted limits and clinical standards required for the various national controls, mastdiscsTM AST are suitable for antibiotic susceptibility studies in laboratories worldwide.

  • Extensive range available from stock to suit all popular clinical applications.
  • Flexible, personalised service manufacturing antibiotic susceptibility discs to customer specification, with low minimum order quantity.
  • All discs are strictly quality controlled for antimicrobial potency and batch uniformity.
  • Discs are available to suit EUCAST, CLSI, BSAC and other laboratory standard recommendations.
  • Inhibition zone diameters can be read by eye or manually using calipers
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Rapid Polymyxin NP

Rapid  Detection of Resistance to Polymyxins

The Rapid Polymyxin NP test has been designed for the detection of Enterobacteriaceae susceptibility and resistance to polymyxins (polymyxin E or Colistin, and polymyxin B) from colonies grown on agar plates. It comes with such direct advantages:

  • Fast results in a 2-3 hours from any enterobacterial species
  • Reliable approach, regardless of the molecular mechanism of resistance (MCR1 and others)
  • Easy to perform, highly specific and sensitive
  • Excellent correlation with the reference broth microdilution method in 24h

Catalogue Number(s)

23000
Product Description: Rapid Polymyxin NP
Number of Tests: 10 test

 

Rapid Polymyxin™ Pseudomonas

Rapid Detection of Resistance to Polymyxins

The Rapid Polymyxin NP test has been designed for the detection of Pseudomonas aeruginosa susceptibility and resistance to polymyxins (Colistin, and polymyxin B).

  • Fast results in 3-4 hours
  • Reliable approach, regardless of the molecular mechanism of resistance (MCR1 and others)
  • Easy to perform, highly specific and sensitive
  • Excellent correlation with the reference broth microdilution method in 24h

 

Catalogue Number(s)

Ref. 23001
Product Description: Rapid Polymyxin PSEUDOMONAS
Number of Tests: 10 tests

Rapid Polymyxin™ Acinetobacter

Rapid Detection of Resistance to Polymyxins

The Rapid Polymyxin NP test has been designed for the detection of Acinetobacter baumannii susceptibility and resistance to polymyxins (Colistin, and polymyxin B) from colonies grown on agar plates. It comes with such direct advantages:

  • Fast results in 3-4 hours
  • Reliable approach, regardless of the molecular mechanism of resistance (MCR1 and others)
  • Easy to perform, highly specific and sensitive
  • Excellent correlation with the reference broth microdilution method in 24h

Catalogue Number(s)

23002
Product Description: Rapid Polymyxin Acinetobacter
Number of Tests: 10 tests

 

Superpolymyxin™

Culture medium for the detection and isolation of Polymyxin-resistant bacteria strains

The SuperPolymyxin™ is a new selective culture medium for the early detection and isolation of Gram-negative bacteria with a reduced susceptibility to polymyxins. It was specially formulated to avoid swarming of Proteus spp. and to prevent contamination by Gram-positive bacteria and Fungi. The SuperPolymyxin will open new perspectives compared to the existing culture media containing high concentrations of colistin and which are known for screening intrinsic PR bacterial species (such as Serratia marcescens and Burkholderia cepacia) but are not adapted for screening isolates with acquired PR that may be of a low level.

  • Screening medium aimed to detect any Polymyxin-Resistant bacteria regardless of its resistance mechanism and of its level
  • Specially formulated to avoid swarming of Proteus spp. (naturally resistant) and to prevent contamination by Gram-positive bacteria and fungi
  • Critical in human medicine for detecting carriers and containing outbreaks due to resistant isolates
  • Useful in veterinary medicine for surveillance surveys
  • Easy to perform and highly sensitive

Catalogue Number(s)

Ref. 23005
Product Description: SUPERPOLYMYXIN
Number of Tests: TBD

Detection, Prevention & Elimination of Contamination in Cell Cultures + PCR cycler validation

Minerva Biolabs GmbH is a leading biotechnology company for the development and marketing of detection & elimination kits for the control of microbial contamination. The company is based in Berlin. Their core competence lies in the control of mycoplasma, bacteria and viruses in cell cultures and biopharmaceuticals. In addition they employ their specialized kits and reagents as PCR diagnostics of respiratory tract infections and for the detection of legionella in water. They regard it as their function to be your highly competent expert in these specialized areas of contamination control, clinical diagnostics and water diagnostics.


The Mycoplasma Detection Kits employ PCR technology for rapid and reliable detection of more
than 110 Mollicutes species, including Mycoplasma, Acholeplasma and Spiroplasma. All relevant
species known as contaminants in various in situ biologicals including cell cultures, culture media, cell
preparations and virus stocks are detectable. One of the different types will fit each individual customer’s
need for a fast, reliable and time-saving routine monitoring!
Mynox® is a biological agent that integrates selectively into the mycoplasma membrane
and compromises its integrity. The result is an osmotic influx that leads to the complete
disintegration of the mycoplasma membrane. With mycoplasma eradicated, cells can
immediately return to their native morphology and normal proliferation rates.


Onar® Bacteria utilizes the polymerase chain reaction (PCR) for highly sensitive detection of
bacterial contamination in various in situ biologicals including cell cultures and virus stocks.
The primer set is specific to a highly conserved region within the 16S ribosomal RNA gene in
the bacteria genome. This allows for detection of all bacteria species tested thus far and usually
encountered as airborne contaminants in cell cultures. Eukaryotic DNA is not amplified by
Onar® Bacteria. The Primer/Nucleotide Reaction Mix already provides an Internal Control DNA as an
essential tool for successfully executed reactions.


ZellShield® protects cell cultures from a broad range of common contaminants.
ZellShield® is active against most intracellular and extracellular growing gram negative and gram
positive bacteria, mycoplasma, protozoon, fungi and yeast.
WaterShield™ protects the water in the reservoir from microbial growth. WaterShield™ can be used in
any kind of laboratory water reservoirs and is compatible with common work surfaces.
Mycoplasma-Off™ is a highly effective solution for cleansing and disinfection of all suitable laboratory
surfaces and apparatus. Mycoplasma-Off™ is outstandingly safe in use possessing not only a
strong mycoplasmacidal effect, but also being effective against bacteria, yeasts and viruses: Mycoplasma-
Off™ has been proven to be effective against bacteria such as Staphylococcus aureus,
Enterococcus hirae, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Candida albicans,
BVDV, Poliovirus, Adenovirus, Polyomavirus SV40, Norovirus and Vacciniavirus.


The PCR Cycler Check™ kits allow a realistic verification of block and air heated PCR cyclers for installation
qualification (IQ), operational qualification (OQ) and performance qualification (PQ) according
to legal requirements, like ISO 17025, EN 45001, ISO 13485, ISO/TS 20836:2007, GLP, GMP and
others.
The PCR Cycler Check™ series provides temperature sensitive PCR reactions to monitor an upper and
lower temperature range in one PCR reaction. The primer sequences and the PCR protocol were designed
to react extremely sensitive on fluctuations of more than 2 °C within the cycler protocol with a drop
out of amplification. This way the temperature homogeneity, the precision of the temperature control
and the timing can be verified. In addition, the pre-adjusted target concentrations are only amplified at
high PCR efficiencies as an additional indicator for accurate temperature control of the thermal cycler.


AquaScreen® is a test system for quantitative detection of water pathogens. AquaScreen® combines
water filtration, lysis of the collected microorganisms, DNA extraction and elution of the DNA in minimal
volumes ready for subsequent PCR analysis.


Food Control™ kit series includes Food Control™ qPCR, Food Control™ LFA and Food Control™ LFA+
PCR kits for fast and reliable detection of foodborne pathogens via real-time PCR or via our proprietary
Lateral Flow Assay (LFA) technology, respectively. Food Control™ qPCR is a diagnostic system for easy
determination of contamination degree in agricultural or food industry via real-time PCR. LFA technology
provides stripes as an easy read-out making DNA gels obsolete and in combination with our LFA+
cartridges and pre-cast chambers provide maximum contamination prevention. Food Control™ kits can
be combined with our optimized DNA extraction system ExtractNow™ Food Control.


We offer our highly effective decontamination products WaterShield™, Lab Clean™, PCR Clean™ and
PCR Clean™ Wipes in order to help you to protect your research. Watershield™ is a disinfection additive
for incubators and water baths and is active against a broad range of typical water contaminations from
bacteria, mycoplasma, protozoan, algae, fungi and yeast. Water exchange is conveniently indicated by
a diminishing blue stain. Lab Clean™ is a large area decontamination solution useful for floors preventing
room-to-room spreading of e.g. DNA carry over or trace amounts of DNA, keeping your lab “DNAload”
low if regularly applied. Finally, protect your PCR experiments from e.g. carry over or DNases and
RNases using PCR Clean™ or PCR Clean™ Wipes highly effective surface decontamination reagents.